DNA sequencing is one of the most important tools used in molecular biology, genetics, genomics, and genetic engineering. Equipped with both a traditional Sanger sequencer (ABI 3730 DNA Analyzer) and a next-generation sequencer (Illumina’s MiSeq), we are capable of sequencing plasmid DNAs, PCR amplicons, mitochondrial genome, transcriptomes of tissue or cell samples, soil microbial community metagenomes, and genome resequencing. For instance, we have re-sequenced the genome of 8 Daphnia pulex populations (see Accession: SAMN02777158 at NCBI’s BioSample database) and sequenced the 16S amplicons of soil microbial metagenome (see SAMN02767911).