The EGSB Utilizes a Well-Equipped Facility that Promotes Cutting-edge Advances in Genomics and System Biology Research
The EGSB has a multifaceted laboratory designed for state-of-the-art genomics, molecular biology, and systems biology research, providing a number of resources that enhance advanced research techniques in these specialized areas.Facilities Overview (5200 Total Square Feet of Laboratory Space)
- 2000 square foot general genomics and molecular biology laboratory.
- 200 square foot cell microinjection facility.
- 3000 square foot zebrafish testing laboratory.
- 1500 square foot fish laboratory
- 1500 square foot zebrafish molecular biology laboratory.
General Genomics and Molecular Biology Laboratory – This laboratory space is equipped with specialized tools used to extract nucleic acids (RNA and DNA), assess nucleic acid quantity / quality, amplify nucleic acids (PCR) and quantify nucleic acid counts individually (qPCR) or globally (microarray or RNAseq). Major laboratory resources include:
- MPure Fast-Prep-24 Tissue Homoginizer (Nucleic Acid and Protein Extraction)
- Various centrifuges (Tissue Extractions, Biomolecule Separations)
- Thermo Scientific NanoDrop One Microvolume UV-Vis Spectrophotometer (Nucleic Acid Quantification)
- Agilent Technologies 2100 Bioanalyzer (Nucleic Acid Quantity / Quality Assessment)
- 4 Live Technologies Thermal-Cyclers (96 well) (PCR and Other Nucleic Acid Biochemistry)
- Agilent Technologies Microarray Hybridization Chamber (Global Gene Expression)
- Agilent Technologies G2505 High-Resolution Microarray Scanner (Global Gene Expression)
- Illumina MiSeq next generation DNA sequencer (Genotyping and Small-scale RNAseq)
- Applied Biosystems (ABI) Prism 7900HT Sequence Detection System (Individual Gene Expression)
- Life Technologies 3500XL genetic analyzer (Individual-Sample DNA Sequencing)
- Multiple -80°C Freezers on back-up generators (Secure Sample Storage / Archive)
- Various Computational Software (genomic, systems biological and bioinformatics analyses)
- Access to the Information Technology Laboratory (ITL) High Performance Computing Facility
- Bioinformatics – sequence assembly, annotations, biological networks and more