The EGSB Utilizes a Well-Equipped Facility that Promotes Cutting-edge Advances in Genomics and System Biology Research

The EGSB has a multifaceted laboratory designed for state-of-the-art genomics, molecular biology, and systems biology research, providing a number of resources that enhance advanced research techniques in these specialized areas.Facilities Overview (5200 Total Square Feet of Laboratory Space)

  • 2000 square foot general genomics and molecular biology laboratory.
  • 200 square foot cell microinjection facility.
  • 3000 square foot zebrafish testing laboratory.
    • 1500 square foot fish laboratory
    • 1500 square foot zebrafish molecular biology laboratory.

 General Genomics and Molecular Biology Laboratory – This laboratory space is equipped with specialized tools used to extract nucleic acids (RNA and DNA), assess nucleic acid quantity / quality, amplify nucleic acids (PCR) and quantify nucleic acid counts individually (qPCR) or globally (microarray or RNAseq).  Major laboratory resources include:

  • MPure Fast-Prep-24 Tissue Homoginizer (Nucleic Acid and Protein Extraction)
  • Various centrifuges (Tissue Extractions, Biomolecule Separations)
  • Thermo Scientific NanoDrop One Microvolume UV-Vis Spectrophotometer (Nucleic Acid Quantification)
  • Agilent Technologies 2100 Bioanalyzer (Nucleic Acid Quantity / Quality Assessment)
  • 4 Live Technologies Thermal-Cyclers (96 well) (PCR and Other Nucleic Acid Biochemistry)
  • Agilent Technologies Microarray Hybridization Chamber (Global Gene Expression)
  • Agilent Technologies G2505 High-Resolution Microarray Scanner (Global Gene Expression)
  • Illumina MiSeq next generation DNA sequencer (Genotyping and Small-scale RNAseq)
  • Applied Biosystems (ABI) Prism 7900HT Sequence Detection System (Individual Gene Expression)
  • Life Technologies 3500XL genetic analyzer (Individual-Sample DNA Sequencing)
  • Multiple -80°C Freezers on back-up generators (Secure Sample Storage / Archive)
  • Various Computational Software (genomic, systems biological and bioinformatics analyses)
  • Access to the Information Technology Laboratory (ITL) High Performance Computing Facility
  • Bioinformatics – sequence assembly, annotations, biological networks and more