Systems Ecotoxicology

The EGSB Team are world leaders in advancing systems toxicology and systems ecotoxicology research demonstrated by numerous high impact publications (see bibliography below). “Systems”-level toxicological research and development integrates responses across multiple levels of biological organization providing holistic interpretations of toxicology / ecotoxicology. Much of the EGSB Team’s work integrates genomics as well as other “-omics” responses to metabolic, cellular, tissue, organ and/or organism-level responses and have been incorporated into adverse outcome pathways (AOPs) for regulatory use.The EGSB has powerful expertise and resources to holistically characterize your chemicals of concern. Contact us for a consultation.

References

• Collier, Z.A., Gust, K.A., Gonzalez-Morales, B., Gong, P., Wilbanks, M.S., Linkov, I., Perkins, E.J., 2016. A weight of evidence assessment approach for adverse outcome pathways. Regul. Toxicol. Pharmacol. 75, 46-57.
• Gong, P., Hong, H., Perkins, E.J., 2015. Ionotropic GABA receptor antagonism-induced adverse outcome pathways for potential neurotoxicity biomarkers. Biomark. Med. 9, 1225-1239.
• Gust KA, Nanduri B, Rawat A, Wilbanks MS, Ang CY, Johnson DR, Pendarvis K, Chen X, Quinn Jr. MJ, Johnson MS, Burgess SC, Perkins EJ (2015) Systems Toxicology Identifies Mechanistic Impacts of 2-amino-4,6-dinitrotoluene (2A-DNT) Exposure in Northern Bobwhite. BMC Genomics. 16:587. doi:10.1186/s12864-015-1798-4.
• Ananthasubramaniam B, McCauley E, Gust KA, Kennedy AJ, Muller EB, Perkins EJ_, Nisbet RM (2015) Relating sub-organismal processes to ecotoxicological and population end points using a bioenergetic model. Ecol Applications. 25:1691–1710. https://dx.doi.org/10.1890/14-0498.1.
• Garcia-Reyero, N., 2015. Are Adverse Outcome Pathways Here to Stay? Environ. Sci. Technol. 49, 3-9.
• Garcia-Reyero, N., Kennedy, A.J., Escalon, B.L., Habib, T., Laird, J.G., Rawat, A., Wiseman, S., Hecker, M., Denslow, N., Steevens, J.A., Perkins, E.J., 2014. Differential effects and potential adverse outcomes of ionic silver and silver nanoparticles in vivo and in vitro. Environ. Sci. Technol. 48, 4546-4555.
• Gust KA, Najar FZ, Habib T, Lotufo GR, Piggot AM, Fouke BW, Laird JG, Wilbanks MS, Rawat A, Indest KJ, Roe BA, Perkins EJ. (2014) Coral-Zooxanthellae Meta-Transcriptomics Reveals Integrated Response to Pollutant Stress. BMC Genomics. 15:591. doi: 10.1186/1471-2164-15-591.
• Wilbanks MS, Gust KA, Atwa S, Sunesara I, Johnson DS, Ang CY, Meyer SA, Perkins EJ (2014) Validation of a genomics-based hypothetical adverse outcome pathway: 2,4-dinitrotoluene perturbs PPAR signaling impairing energy metabolism and decreasing exercise endurance, Toxicol Sci.141(1):44-58. doi: 10.1093/toxsci/kfu104.
• Perkins, E.J., Ankley, G.T., Crofton, K.M., Garcia-Reyero, N., LaLone, C.A., Johnson, M.S., Tietge, J.E., Villeneuve, D.L., 2013. Current perspectives on the use of alternative species in human health and ecological hazard assessments. Environ. Health Perspect. 121, 1002-1010.
• Stanley, J.K., Perkins, E.J., Habib, T., Sims, J.G., Chappell, P., Escalon, B.L., Wilbanks, M., Garcia-Reyero, N., 2013. The good, the bad, and the toxic: approaching hormesis in Daphnia magna exposed to an energetic compound. Environ. Sci. Technol. 47, 9424-9433.
• Warner CM, Gust KA*, Stanley JK, Habib T, Wilbanks MS, Garcia-Reyero N, Perkins EJ (2012) A Systems Toxicology Approach to Elucidate the Mechanisms Involved in RDX Species-Specific Sensitivity. Environ Sci Technol. 46:7790−7798.
• Chaitankar V, Ghosh P, Elasri, MO, Gust KA, Perkins EJ (2012) Genome scale inference of transcriptional regulatory networks using mutual information on complex interactions. Proceedings of the ACM Conference on Bioinformatics, Computational Biology and Biomedicine. DOI:10.1145/2382936.2383051.
• Deng, Y., Meyer, S.A., Guan, X., Escalon, B.L., Ai, J., Wilbanks, M.S., Welti, R., Garcia-Reyero, N., Perkins, E.J., 2011. Analysis of common and specific mechanisms of liver function affected by nitrotoluene compounds. PLoS One 6, e14662.
• Gust KA, Brasfield SM, Stanley JK, Wilbanks MS, Chappell P, Perkins EJ, Lotufo GR, Lance RF (2011) Genomic investigation of year long and multi-generational exposures of fathead minnow to the munitions compound RDX. Environ Toxicol Chem. 30(8):1852-1864.
• Garcia-Reyero N, Habib T, Pirooznia M, Gust KA, Gong P, Warner C, Wilbanks MS, Perkins E (2011) Conserved toxic responses across divergent phylogenetic lineages: A Meta-analysis of the neurotoxic effects of RDX among multiple species using toxicogenomics. Ecotoxicology 20(3):580-594.
• Gust KA, Wilbanks MS, Guan X, Pirooznia M, Habib T, Yoo L, Wintz H, Vulpe CD, Perkins EJ (2011) Investigations of transcript expression in fathead minnow (Pimephales promelas) brain tissue reveal toxicological impacts of RDX exposure. Aquat Toxicol. 101(1):135-45.
• Deng, Y., Johnson, D.R., Guan, X., Ang, C.Y., Ai, J., Perkins, E.J., 2010. In vitro gene regulatory networks predict in vivo function of liver. BMC Syst. Biol. 4, 153.
• Rawat A, Gust KA*, Deng Y, Garcia-Reyero N, Quinn Jr. MJ, Johnson MS, Indest K, Elasri MO, Perkins EJ (2010) From raw materials to validated system: The construction of a genomic library and microarray to interpret systemic perturbations in Northern bobwhite. Physiol Genomics. 42:219-235.
• Rawat A, Gust KA, Elasri MO, Perkins EJ (2010) Quail Genomics: a knowledgebase for Northern bobwhite. BMC Bioinformatics. 11(Suppl 6):S13.
• Gust KA, Pirooznia M, Quinn Jr. MJ, Johnson MS, Escalon, BL, Indest KJ, Guan X, Clarke J, Deng Y, Gong P, Perkins EJ (2009) Neurotoxicogenomic Investigations to Assess Mechanisms of Action of the Munitions Constituents RDX and 2,6-DNT in Northern Bobwhite (Colinus virginianus) Toxicol Sci 110(1):168–180.